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Andrew N. Lane

James Graham Brown Endowed Chair of Structural Biology
Professor of Medicine
Leader, Structural Biology Program

Andrew N. Lane
Specialties: Structural Biology, Biophysics and Metabolism
Address: CTR Building, 505 South Hancock Street, Louisville, KY 40202
Phone: (502) 852-3067


1972-1975 B.Sc., University College London, England, Biochemistry
1975-1979 Ph.D., University College London, England, Biochemistry
1979-1983 Post doctoral Fellow, Biophysical Chemistry, Biozentrum, Basel, Switzerland
1983-1986 Post doctoral Fellow, Stanford Magnetic Resonance Laboratory, Stanford University

Research and Professional Experience

1986-1994 Staff Scientist, National Institute for Medical Research, London, UK
1994-2001 Senior Staff Scientist, National Institute for Medical Research, London
1997-2000 Committee British Biophysical Society
2001-present Professor of Medicine, University of Louisville
2001-present Director, James Graham Brown Cancer Center NMR Facility
2002-present Leader, Structural Biology Program – James Graham Brown Cancer Center
2002-present Professor of Chemistry (secondary), University of Louisville
2002-present Professor Biochemistry & Molecular Biology (secondary), University of Louisville
2005-present Associate Director (NMR) Center for Regulatory Environmental Analytical Metabolomics
Editorial Board: Nucleic Acids Research
Editorial Board: Journal of Structural and Functional Genomics
Editor, Biophysics: Central European Journal of Biology
Editorial Board: Metabolomics
Editorial Board: Cancer & Metabolism

Awards and Honors

Honorary Research Fellow, Department of Biochemistry and Molecular Biology, University College London
1983-1985 EMBO Fellow, Stanford University
James Graham Brown Endowed Chair of Structural Biology, University of Louisville
Mint Jubilee Scientist of the Year 2009 Metabolomics Team

Research Interests

My research is divided into structural and biophysical analyses of macromolecules (DNA and proteins) and their interactions relevant to cancer biology; and stable isotope resolved metabolomics (SIRM) as applied to understanding cancer pathology, and as an approach for target discovery and validation.

Publications (recent or significant)

Lane, A.N. & Jenkins, T.C. (2000) "Thermodynamics of nucleic acids and their interactions with ligands". Q. Rev. Biophys. 33, 255-306 (invited review)

Lane, A.N. (2001) "Determination of the structures of drug-DNA complexes in solution by NMR". Methods Enzymol. 340, 252-281

Alexandrovich, A., Czisch, M., Frenkiel, T.A., Moolenaar, G.F., Goosen, N., Sanderson, M.R.& Lane, A.N. (2001) Quaternary structure, thermodynamics and dynamics of the C-terminal domain of UvrB. J. Biol. Ster. Dyn. 19, 219-236

Lane, A.N., Kelly, G., Ramos, A. & Frenkiel, T.A. (2001) Determining binding sites in protein-nucleic acid complexes by cross-saturation. J. Biomolec. NMR 21, 127-139

Gopal, B., Haire, L.F., Gamblin, S.J., Dodson, E.J., Lane, A.N., Papavinasundaram, K.G., Colston, M.J. & Dodson, G. (2001) Crystal structure of the transcription elongation/antitermination factor NusA from Mycobacterum tuberculosis at 1.7 Å resolution. J. Mol. Biol. 314, 1087-1095

Nair, M., McIntosh, P.B., Frenkiel, T.A., Kelly, G., Taylor,I.A., Smerdon, S.J., & Lane A.N. (2003) NMR Structure of the DNA-binding Domain of the Cell Cycle protein, Mbp1 from Saccharomyces cerevisiae. Biochemistry, 42, 1266 –1273

Gyi, J.I., Gao, D., Conn, G.L., Trent, J.O., Brown, T. & Lane, A.N. (2003) The solution structure of a DNA·RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications. Nucl. Acids Res 31, 2683-2693.

Ramos, A., Lane, A.N., Hollingworth, D. & Fan, T.W-M.(2004) NMR determination of the secondary structure of the selenocysteine insertion element. Nucl. Acids Res. 32, 1746-1755.

Sengodagounder Arumugam, R.D. Gray & A. N. Lane (2005) 1H, 15N and 13C assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa. J. Biomolec. NMR. 11, 265-266.

Lane, A.N. & Sengodagounder Arumugam (2005). Improving NMR sensitivity in room temperature and cooled probes with dipolar ions. J. Magn. Reson. 173, 339-343.

Booth, J., Brown, T., Vadhia, S.J., Lack, O., Cummins,W.J., Trent, J.O., & Lane A.N. (2005) Determining the origin of the stabilization of DNA by 5-aminopropynylation of pyrimidines. Biochemistry. 44, 4710-4719

Fan, T. W-M., Bandura, L.L., Lane, A.N. & Higashi, R.M. (2005) Metabolomics-Edited Transcriptomics Analysis of Se Anticancer Action in human lung cancer cells. Metabolomics 1, 325-339

Telang S. Yalcin A., Clem A. L.,Bucala R., Lane A. N., Eaton, J. W., & Chesney J. A. (2006) Ras Transformation Requires Metabolic Control by 6-Phosphofructo-2-Kinase. Oncogene 25: 7225-7234

Lane, A.N. & Fan, T. W-M. (2007) Determination of positional isotopomers in metabolites. Metabolomics 3, 79-86

Kurtoglu, M., Gao, N., Maher, J.C., Lehrman, M.A., Wangpaichitr, M.,Savaraj, N., Lane, A.N. and Lampidis, T.J. . (2007) Under normoxia 2-deoxy-D-Glucose, but not 2- fluoro-deoxy-D-glucose, kills select tumor cells by interfering with N-linked glycosylation. Molecular Cancer Therapeutics 6, 3049-3058.

Telang, S., Lane, A.N., Nelson, K.K. Arumugam, S. &, Chesney, J.A.(2007) The oncoprotein H- RasV12 increases mitochondrial metabolism Molec. Cancer. 6, 77

El-Sagheer, R. Kumar, S. Findlow, J. Werner, A.N. Lane and T. Brown. (2008) A Very Stable Cyclic DNA Miniduplex with Just Two Base Pairs. ChemBioChem 9, 50-52.

Clem, B.F., Telang, S., Clem, A., Yalcin, A., Meier, J., Simmons, A., Rasku, M..A, Arumugam, S., Dean, W.L., Eaton, J. Lane, A.N., Trent, J.O. and Chesney. J.A. (2008) “Small Molecule Inhibition of 6-Phosphofructo-2-Kinase Activity Suppresses Glycolytic Flux and Tumor Growth” Molecular Cancer Therapeutics. 7, 110-120

Arumugam, S., Gray, R.D., Lane, A.N. (2008) Solution structure of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa: mechanism of inhibition J. Biomolec. NMR 40, 213-217.

Deleeuw, L., Tchernatynskaia, A.V. and Lane, A.N. (2008) Thermodynamics and specificity of the Mbp1-DNA Interactions. Biochemistry 47, 6378-6385

Lane, A.N., Fan, T-WM. & Higashi, R.M. (2008) “Isotopomer-based metabolomic analysis by NMR and mass spectrometry". Methods in Cell Biology, 84, 541-588.

Fan, T.W-M. & Lane, A.N. (2008) Structure-based profiling of Metabolites and Isotopomers by NMR. Prog. NMR Spectrosc. 52, 69-117

Lane, A.N., Fan. T. W-M. and Higashi, R.M. (2008) “Stable isotope assisted metabolomics in cancer research”. IUBMB Life 60, 124-129

Fan, T. W-M., Kucia M., Jankowski, K., Higashi, R.M., Rataczjak, M.Z., Rataczjak, J, & Lane, A.N. (2008) Proliferating Rhabdomyosarcoma cells shows an energy producing anabolic metabolic phenotype compared with Primary Myocytes Molecular Cancer 7:79.

Thornburg, J.M., Nelson, K.K., Lane, A.N., Arumugam, S., Simmons, A. Eaton, J.W., Telang, S., & Chesney, J. (2008) Targeting Aspartate Aminotransferase in Breast Cancer. Breast Cancer Res. 10:R84

Lane, A.N., Chaires, J.B., Gray, R.D. & Trent, J.O. (2008)  “Stability and kinetics of G-quartet structures” Nucl. Acids Res. 36, 5482-5515

Lane, A.N., Fan, T. W-M. & Higashi, R.M. (2009) Metabolic acidosis and the importance of balanced equations. Metabolomics 5, 163-165

Fan, T. W-M; Lane, A. N; Higashi, M.; Bousamra III, M.; Kloecker, G.;. Miller, D. M. (2009) Erlotinib-sensitive and resistant lung tumors show radically different metabolic profiles. Exp. Molec. Pathol. 87, 83-86

Fan, T.WM., Lane, A.N., Higashi, R.M., Farag, M.A., Gao, H., Bousamra, M. & Miller, D.M. (2009) Altered Regulation of Metabolic Pathways in Human Lung Cancer Discerned by 13C Stable Isotope-Resolved Metabolomics (SIRM). Molecular Cancer. 8:41

Yalcin, A., Clem, B.F., Simmons, S., Lane, A.N, Nelson, K.K., Clem, A.L., Brock, E., Siow, D., Wattenberg, B., Telang, S. & Chesney, J. (2009) Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases. J. Biol. Chem. 284: 24223 - 24232.

Lane, A.N. , Fan, T. W-M., Xie, X., Moseley, H.N. & Higashi, R.M.(2009) Stable isotope analysis of lipid biosynthesis by high resolution mass spectrometry and NMR. Anal. Chim. Acta. 651, 201-208.

Chernatynskaya, A.V. Deleeuw, L. Trent, J.O., Brown, T. and Lane, A.N. (2009) Structural analysis of the DNA target site and its interaction with Mbp1. Org.Biomolec. Chem. 7, 4981-4991.

A Yalcin, B Clem, S Makoni, A Clem, K Nelson, J Thornburg, D Siow, A N Lane, S E Brock, U Goswami, Eaton, J.W., Telang, S. & Chesney, J. (2010) Selective inhibition of choline kinase simultaneously attenuates MAPK and PI3K/AKT signaling. Oncogene 29, 139–149

Dailey, M.M., Miller, M.C., Bates, P.J., Lane, A.N., Trent, J.O. (2010) Resolution and characterization of the structural polymorphism of a single quadruplex-forming sequence. Nucl. Acids Res. 38, 4877-4888.

Fan, T. W-M. Yuan, P., Lane, A.N., Higashi, R.M. Wang, Y., Hamidi, A., Zhou, R., Guitart-Navarro, X., Chen, G., Manji, H.K., Kaddurah-Daouk, R. (2010) Stable Isotope Resolved Metabolomic Analysis of Lithium Effects on Glial-Neuronal Interactions. Metabolomics 6, 165 – 179.

Arumugam, S., Miller, M.C., Maliekal, J., Trent, J.O., and Lane, A.N. (2010) NMR Structure Note: Solution structure of the RBD1,2 domains from human nucleolin. J. Biomolec. NMR 47, 79-83.

Miller, M.C., Buscaglia, R., Chaires, J.B., Lane, A.N., Trent, J.O. (2010) Hydration determines the G-quadruplex stability and conformation of the human telomere 3’ sequence d(A(GGGTTA)3GGG)). J.Am.Chem. Soc. 132, 17105-17107.

Fan, T. W-M., Lane, A.N., Higashi, R.M., Yan, J. (2011) Stable Isotope Resolved Metabolomics of Lung Cancer in a SCID Mouse Model. Metabolomics 7, 257-269

Fan, T.W-M. & Lane, A.N. (2011) NMR-based Stable Isotope Resolved Metabolomics in Systems Biochemistry. J. Biomolec. NMR Sp. Edn. 49:267–280

Lane, A.N., Fan, T. W-M., Bousamra II, M., Higashi, R.M., Yan, J., Miller, D.M. (2011) Stable Isotope-Resolved Metabolomics (SIRM) in Cancer Research with Clinical Application to Non-Small Cell Lung Cancer. Omics 15, 173-182.

Moseley, H.N.B., Lane, A.N., Belshoff, A.C, Higashi, R.M. Fan, W. W-M. (2011) Non-Steady State Modeling of UDP-GlcNAc Biosynthesis Enabled by Stable Isotope Resolved Metabolomics. BMC Biology. 9:37.

Fan, T. W-M., Tan, J., McKinney, M.M., Lane, A.N. (2011) Stable Isotope Resolved Metabolomics Analysis of Ribonucleotide Metabolism in Lung Cancer Cells. Metabolomics. 8, 517-527 

Lane, A.N. (2012) The stability of intramolecular DNA quadruplexes compared with other macromolecules. Biochimie. 94:277-86

Le, A., Lane, A.N., Hamaker, M., Bose, S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. L.J., Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., and Dang, C.V. (2012) Myc induction of hypoxic glutamine metabolism and a glucose-independent TCA cycle in human B lymphocytes. Cell Metabolism. 15, 110-121 

Lorkiewicz, P., Higashi, R.M., Lane, A.N., Fan, T. W-M. A Method for nucleotide detection using direct-infusion FT-ICR MS for the purpose of Stable Isotope Resolved Metabolomics (SIRM) analysis. Metabolomics  8, 930-939

Liu, W., Le, A., Fan, T. W-M., Lane, A.N., Dang, C.V., Phang, J.M. (2012) The reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses to c-MYC. PNAS 109:8983-8988      

Fan, T.W-M., Higashi, R.M. & Lane, A.N. (2012) “Overview of Metabolomics” in “The Handbook of Metabolomics Methods in Pharmacology and Toxicology, vol. 17” Humana Press 2012. Ch 1.DOI 10.1007/978-1-61779-618-0_4.

Lane, A.N. (2012) “NMR applications in metabolomics” in “The Handbook of Metabolomics Methods in Pharmacology and Toxicology, vol. 17” Humana Press 2012. Ch 6. DOI 10.1007/978-1-61779-618-0_4.

Poulo, J.M., Elston, T., Lane, A.N., Macdonald, J.M. and Cascante, M. (2012) Introduction to Metabolic Control Analysis (MCA). in “The Handbook of Metabolomics Methods in Pharmacology and Toxicology, vol. 17” Humana Press 2012. Ch 9. DOI 10.1007/978-1-61779-618-0_4.

Bousamra, M., Day, J., Fan, T. W-M., Higashi, R.M., Kloecker, G, Lane, A.N., Miller, D.M. (2012) “Clinical aspects of metabolomics “ in “The Handbook of Metabolomics Methods in Pharmacology and Toxicology, vol. 17” Humana Press 2012. Ch 3. DOI 10.1007/978-1-61779-618-0_4.

Maldonado, C., Pushpakumar, S.B.,  Arumugam, S., Perez-Abadio, G.,  Lane, A.N.(2013)

Administration of Exogenous ATP to Ischemic Skeletal Muscle Induces an Energy Sparing Effect that Involves Adenosine Receptors. Journal of Surgical Research in press. DOI: 10.1016/j.jss.2012.06.033

Fan, T. W-M., Lane, A.N. (2013) “Assignment strategies for NMR spectroscopic signals in metabolomics research “ in Methodologies for Metabolomics: Experimental Strategies and Techniques, Lutz, Sweedler & Wever, Eds. Cambridge University Press.   ISBN:9780521765909    Ch 24, pp 525  

Lane A.N. (2013) Bacterial repression: The Lac and Trp Systems. Online Encyclopedia of Biophysics, Pp168-172 Springer Verlag. DOI 10.1007/978-3-642-16712-6.

Reynolds, M.R., Lane, A.N., Kemp, S., Liu, Y., Hill, B., Dean, D.C., Clem, B.F. (2013) Control of Glutamine Metabolism By the Tumor Suppressor Rb. Oncogene in press

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