You are here: Home → Research Activities → Andrew Lane - Associate Director of NMR Development

Andrew Lane - Associate Director of NMR Development

Andrew N. Lane, PhD

Endowed Professor

James Graham Brown Cancer Center, Suite 431

529 S. Jackson Street

University of Louisville

Louisville, KY 40202 USA.

email: anlane01@gwise.louisville.edu

Areas of Reserach Interest:

    • Analysis of enzyme structure and mechanisms by electronic spectroscopy (circular dichroism, fluorescence and uv-vis), solution scattering methods (dynamic light scattering, small angle scattering);

    • Stopped-flow reaction kinetics, thermodynamics and hydrodynamics.

    • Protein expression and purification and stable isotope labelling.

    • 2D and 3D heteronuclear NMR for the determination of structure, dynamics and interactions of nucleic acids and proteins in solution.

    • Computer programming for data analysis and simulation of NMR relaxation behaviour in complex spin systems for structure determination.

    • Use of stable isotope tracers for analysing metabolic regulation in vitro  and in vivo  by NMR.

 

Recent Publications.

Nair M., McIntosh, P.B., Frenkiel, T.A., Kelly, G., Taylor,I.A., Smerdon, S.J., & Lane A.N. (2003) NMR Structure of the DNA-binding Domain of the Cell Cycle protein, Mbp1 from Saccharomyces cerevisiae.  Biochemistry, 42 1266 –1273

Gyi,  J.I., Gao, D., Conn, G.L., Trent, J.O.,  Brown, T. & Lane,  A.N. (2003) The solution structure of a DNA·RNA duplex containing 5-propynyl U and C; comparison with 5-Me modifications. Nucl. Acids Res 31, 2683-2693.

Fan, T. W-M. Lane, A.N. & Higashi, R.M. (2003) In Vivo and In Vitro Metabolomic Analysis of Anaerobic Rice Coleoptiles Revealed Unexpected Pathways. Russ. J. Plant Physiol. 50, 787-793

Fan, T.W-M., Higashi, R.M. & Lane, A.N. (2004) "A Rapid Electrophoretic Profiling Method for Thiol-Rich Peptides and Proteins". Phytochem. Anal. 15, 175-183

Fan, T. W-M., Lane, A.N. & Higashi, R.M., (2004) The Promise of Metabolomics in Cancer Molecular Therapeutics. Current Opin. Molec. Ther. 6:584-592   

Fan, T. W-M., Lane, A.N., Chekmenev, E., Wittebort, R.H.  & Higashi, R. M. (2004) “Determination of the composition and physical properties of peptides in soil organic matter” J. Peptide Res. 63 , 253-264

Ramos, A., Lane, A.N., Hollingworth, D. & Fan, T.W-M.(2004) NMR determination of the secondary structure of the selenocysteine insertion element. Nucl.  Acids Res. 32, 1746-1755.

Sengodagounder  Arumugam, R.D. Gray & A. N. Lane (2005) 1H, 15N and 13C assignments of the alkaline proteinase inhibitor APRin from Pseudomonas aeruginosa. J. Biomolec. NMR. 11, 265-266

Lane, A.N.  & Sengodagounder Arumugam (2005). Improving NMR sensitivity in room temperature and cooled probes with dipolar ions. J. Magn. Reson. 173, 339-343.

Booth, J., Brown, T., Vadhia, S.J., Lack, O., Cummins,W.J., Trent, J.O.,  & Lane A.N. .(2005) Determining the origin of the stabilization of DNA by 5-aminopropynylation of pyrimidines.   Biochemistry. 44,4710-4719

Fan, T. W-M., Bandura, L.L., Lane, A.N. & Higashi, R.M. (2005) Metabolomics-Edited Transcriptomics Analysis of Se Anticancer Action in human lung cancer cells. Metabolomics . 1, 325-339

Fan, T. W-M.,Bandura, L.L.  Lane, A.N. & Higashi, R.M., (2006) “ Integrating Genomics and Metabolomics for Probing Se Anticancer Mechanisms” Drug Metabolism Reviews 38, 1-25

Telang S. Yalcin A., Clem A. L.,Bucala R., Lane A. N., Eaton, J. W., & Chesney J. A. (2006) Ras Transformation Requires Metabolic Control by 6-Phosphofructo-2-Kinase. Oncogene 25: 7225-7234

Lane, A.N. & Fan, T. W-M. (2007) Determination  of positional isotopomers in metabolites. Metabolomics In Press

Lane, A.N., Fan, T-WM.  & Higashi, R.M. (2007)  “Isotopomer-based metabolomic analysis by NMR and mass spectrometry". Biophysical Tools for Biologists, Volume 1, edited by Drs. John J. Correia and H. William Detrich. Ch 22.   In press

Fan, T.W-M. & Lane, A.N. (2007) Isotope editing in metabolomics analyses. Prog. NMR Spectrosc. (invited review)

 

 

Document Actions