Protein Expression and Purification Core

Actively pursuing Louisville Research Core designation

About Us

The Protein Expression and Purification Core provides purified proteins through expression optimization, large-scale expression, isotopic labeling, and protein refolding services.

The Protein Expression and Purification Core is a core component of the Drug Discovery Platform.

Services

  • Expression Vector Construction
  • Growth Media Preparation
  • Transformation
  • SDS-PAGE Gel Service
  • Expression Optimization
  • Large-scale Expression
  • Isotopically labeled Protein Expression for NMR
  • Cell extract preparation
  • Protein Purification
  • Protein Refolding
  • Tag Removal

Serving

  • UofL Researchers
  • External-Academic
  • External-Industry

Core Acknowledgement

Researchers are strongly encouraged to acknowledge the use of this core facility in publications and presentations. Doing so documents the role of shared infrastructure in advancing UofL research and supports the core’s continued operation and growth. 

Consider using the suggested language below when making acknowledgements in your work:

This [publication, project, or research] was made possible, in part, by the Protein Expression and Purification Core [RRID:SCR_026516] at the University of Louisville. 

Core Personnel

T. Michael Sabo 
Director, Protein Expression and Purification Core

Anne Carenbauer
Research Program Manager II

Protein Expression and Purification Core

Brown Cancer Center

Website about

Location

Clinical & Translational Research Building, Room 233B-D